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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in lots of tumors, which leads to chromosome instability, and so might contribute to tumorigenesis. This protein was observed to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a crucial part in selling cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been described. Two added splice variants ended up noted but in-depth nucleotide sequence information is not yet out there.
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Blocking peptides are peptides that bind exclusively to your focus on antibody and block antibody binding. These peptide usually includes the epitope acknowledged because of the antibody.
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This information outlines even more achievable optimization of mobile and immunoassay parameters website to be certain the very best success are...
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This manual outlines further more doable optimization of cellular and immunoassay parameters to be certain the best possible success are...
Antibodies bound to the blocking peptide no more bind towards the epitope to the concentrate on protein. By comparing the staining within the blocked antibody compared to the antibody by yourself, you can see which staining is particular.
Goal Information and facts Cyclin E belongs into the extremely conserved cyclin relatives, whose users are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins function as regulators of CDK kinases. Distinct cyclins show distinctive expression and degradation patterns which contribute for the temporal coordination of every mitotic celebration. Cyclin E sorts a posh with and functions as a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates for the G1-S period boundary which is degraded as cells progress via S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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